Welcome to the Exchange

The FlowJo Exchange is a repository that allows users to upload annotated scripts and download shared scripts. Plugins are a new addition to the Exchange that allow users to create their own extended functionality for the FlowJo application. The open plugin architecture introduced with 10.1r7 allows FlowJo users access to cutting-edge tools that can be integrated into their current FlowJo analysis workflows, so any tool can be integrated into FlowJo.

Don't worry, we are pretty obsessive about documentation. Each script, plugin, or visualization we provide includes instructions that step you through how to use it, and we urge others who post to do the same. No JavaScript, HTML, or black belt skills are required.

Featured: FlowClean Plugin

The FlowClean plugin is an algorithm that automatically identifies and flags fluorescence anomalies in flow cytometry data so that these can be easily removed. The FlowClean algorithm utilizes the R statistical computing environment and integrates FlowClean’s functionality into FlowJo. The algorithm performs calculations on the provided samples, and creates a new population with fluorescence anomalies removed.

Note: FlowClean is a third-party plugin. Support for this plugin may be limited.

Download the plugin now
View the plugin documentation

Tool types

There are three types of tools here, which can all be used with FlowJo.

1. Plugins (NEW)

Yeah, we can cluster that. If you have an algorithm or application that needs to do something upon opening or saving, OR you would like exported population data and user-specified parameters passed to your application, we can do that. In fact, we can bring the clusters back into FlowJo so everyone can use what you have written. To learn more, please see our FlowJo for developers page (coming soon).

Note: You must be running FlowJo version 10.1r7 or higher to use plugins. Earlier versions do not support plugins.

Example Plugins

See these examples for plugin development and documentation done well.

How to Install FlowJo Plugins

The plugin should now be ready for use. Most plugins are accessed from the Workspace ribbon, Populations band, Plugins task. See the User Guide section of the plugin’s documentation for instructions on how to use the newly-installed plugin.

Available Plugins

FlowClean: Automatically identifies and flags fluorescence anomalies in flow cytometry data so that these can be easily removed. This algorithm uses the R statistical computing environment.
Image credit: Julieta Felix, The Noun Project, Creative Commons license.
Email Notification: Sends auto-generated email notifications when a FlowJo client workspace is saved. This plugin promotes communication about changes to the FlowJo workspace. Email notifications may be sent to recipients when the FlowJo workspace is saved in the FlowJo client.
Image credit: Jim Slatton, The Noun Project, Creative Commons license.
Auto-Gating: Allows FlowJo users to make distinct gates around their samples based on child-parent percentages. This plugin generates an optimal sub-population that matches a user's specified percentage.
Image credit: Alfredo Hernandez, The Noun Project, Creative Commons license.
OPTICS: Allows users to perform density-based reordering and cluster detection on populations within FlowJo. This population plugin performs density-based reordering and cluster detection to extract the clusters present in a population node. The user may select what parameters they wish to cluster the data on.
Image credit: Delwar Hossain, The Noun Project, Creative Commons license.
AWS-S3: Uploads FlowJo workspace files to an AWS S3 server. Using the AWS S3 service with FlowJo gives one the ability to upload and download workspaces as a remote backup or share workspace environments with others. The user attaches his or her workspace to a specific S3 bucket, and the workspace is uploaded to this bucket when the workspace is saved.
Image credit: ProSymbols, The Noun Project, Creative Commons license.
LIMS Integrator: Provides PostgreSQL integration with FlowJo for lab management purposes. This plugin allows for the integration of user information associated with a FlowJo workspace and a PostgreSQL database. Except for the initial setup of a PostgreSQL server, all other setup is handled through the plugin, such as database and table creation.
Image credit: Alfredo Hernandez, The Noun Project, Creative Commons license.

2. Scripts

FlowJo's script editor is integrated directly into the core of FlowJo, and gives real-time access to statistics, gates, groups, and metadata to create custom analysis tools within FlowJo. This access makes it easy for:

Scripts produced in the Script editor can be saved as text files, shared, and run by anyone in three clicks by loading them into a FlowJo workspace and clicking Run.

Available Scripts

Indexed Sorting: Compiles index sorting keywords and creates a gate for each cell, with the well location as the name.
Image credit: Hans Paul Mösl Junior, The Noun Project, Creative Commons license.

Basic Loop: Basic loop tool in JavaScript to initialize a new project from.
Image credit: Rflor, The Noun Project, Creative Commons license.

Collection Order Sort: Creates a sortable keyword based on day-month-year metadata.
Image credit: Demograph, The Noun Project, Creative Commons license.

Control Based Inclusion Gating: Automatically creates "positive gates" using control samples' properties.
Image credit: The Noun Project, Creative Commons license.

Relative Gate: Creates a gate relative to a pre-defined (negative) population.
Image credit: Rflor, The Noun Project, Creative Commons license.

3. Visualizations

Some of us spend our weekend playing with visualizations (yes, seriously), and we'll post some here with some instructions for use.

Need to cite the Exchange?

If you use these tools in your research, please give credit as follows:

J. Quinn, S. Duncan, M. Golden, M. Swindle, S. Weiss, M. Stadnisky. FlowJo Exchange : A means of meeting the computational needs of the flow community. CYTO 2015: XXX Congress of the International Society for the Advancement of Cytometry. Glasgow, Scotland. June 2015.

...and please be sure to let people know what script you used.

Need Help?

Drop us a line at exchange@flowjo.com or techsupport@flowjo.com.