The FlowJo Exchange is a repository maintained by FlowJo, LLC that allows users to upload annotated scripts and download shared scripts. Plugins are a new addition to the Exchange that allow users to create their own extended functionality for the FlowJo® application. The open plugin architecture introduced with 10.1r7 allows FlowJo users access to cutting-edge tools that can be integrated into their current FlowJo analysis workflows, so any tool can be integrated into FlowJo.
For researchers wishing to calibrate parameters using MESF beads, this tool will automatically detect peaks, and allow users to assign known molecular equivalents to those control peaks. The tool will then perform a linear regression between the observed peak fluorescence intensities and manufacturer provided molecular values, in order to generate a corresponding calibrated derived parameter.
This popular quantitation allows users to easily gauge the number of molecules associated with a given population, or even a single cell.
||Download the plugin, documentation and demo data file now!|
There are four types of tools here, which can all be used with FlowJo:
Yeah, we can cluster that. If you have an algorithm or application that needs to do something upon opening or saving, or you would like exported population data and user-specified parameters passed to your application, we can do that. In fact, we can bring the clusters back into FlowJo so everyone can use what you have written. To learn more, please see our FlowJo for Developers page (coming soon).
Note: You must be running FlowJo version 10.1r7 or higher to use plugins. Earlier versions do not support plugins.
See these examples for plugin development and documentation done well.
FlowJo's Script Editor is integrated directly into the core of FlowJo, and gives real-time access to statistics, gates, groups, and metadata to create custom analysis tools within FlowJo. This access makes it easy for:
Scripts produced in the Script Editor can be saved as text files, shared, and run by anyone in three clicks by loading them into a FlowJo workspace and clicking Run.
Some of us spend our weekend playing with visualizations (yes, seriously), and we'll post some here with some instructions for use.
The plugins listed below are included with FlowJo v10.1r7 and later. We include them here in case you need to update or re-install them. They can be installed in the same manner as any other plugin—see How to Install FlowJo Plugins above or our plugin documentation for details.
If you use these tools in your research, please give credit as follows:
J. Quinn, S. Duncan, M. Golden, M. Swindle, S. Weiss, M. Stadnisky. FlowJo Exchange : A means of meeting the computational needs of the flow community. CYTO 2015: XXX Congress of the International Society for the Advancement of Cytometry. Glasgow, Scotland. June 2015.
...and please be sure to let people know what script you used.
Drop us a line at email@example.com or firstname.lastname@example.org.